mouse thymoma el4 cells  (ATCC)

Journal: General and comparative endocrinology

Article Title: Thyroid stimulating hormone β-subunit splice variant is expressed in all fractional subsets of bone marrow hematopoietic cells and peripheral blood leukocytes and is modulated during bacterial infection

doi: 10.1016/j.ygcen.2020.113495

Figure Lengend Snippet: Expression of TSHβv in MLN cells from normal mice. (A) Scattergram of cells defined by forward scatter (FSC) and side scatter (SSC) showing three distinct populations consisting of lymphoid cells (gate 1), monocytes (gate 2), and granulocytes (gate 3). Intracellular expression of TSHβv in (B) lymphoid cells, (C) monocytes, (D) granulocytes, (E) CD3+ T cells, and (F) CD19+ B cells. Expression of (G) CD3 on EL4 T cells, and (H) intracellular TSHβv expression in EL4 T cells. PCR analysis of TRHR, TSHβv, and TSHβ gene expression in the mouse (I) AM macrophage cell line, and (J) EL4 T cells. CD138 expression on MLN (K) lymphoid cells, (L) monocytes, and (M) granulocytes, mfi, mean fluorescence intensity.

Article Snippet: EL4 mouse T cell thymoma cells (TIB-39) were purchased from American Type Culture Collection, Manassas, VA.

Techniques: Expressing, Gene Expression, Fluorescence